The interplay of low H2O2 doses, lytic cyanophage, and Microcystis aeruginosa: Implications for cyanobacterial bloom control and microcystin production/release.

Low H2O2 doses can suppress cyanobacterial blooms without damaging non-target species but enable undesirable regrowth. Besides, the role of cyanophage in preventing regrowth after low H2O2 exposure remains unclear. Applying phages to cyanobacteria pre-exposed to low H2O2 in early growth stages may improve host removal and reduce microcystin (MC) production/release. Lytic cyanophage MDM-1 with a 172 PFU/cell burst size, 2-day short latent period against MCs-producing Microcystis, shows high H2O2 stability. Low H2O2 (1 to 2.5 mg/L) doses significantly (p < 0.05) inhibited Microcystis aeruginosa growth rate, biofilm and MCs concentration reduction in a dose-dependent manner but regrowth occurred at all concentrations. Phage treatment eliminated cells without H2O2 pretreatment within 3 days and reduced MC production. H2O2-pretreated M. aeruginosa cells altered the phage dynamics, affecting adsorption, latency, production, and cell lysis in response to H2O2-induced oxidative stress. At 1.5 mg H2O2/L pretreatment, cells were eliminated with reduced MC production, like untreated cells. H2O2 pretreatment with 2.0 and 2.5 mg/L resulted in an extension of the phage absorption phase and the latent period. This was accompanied by a reduction in lysis efficacy, attributed to the increased ROS production. At 2.5 mg H2O2/L, 17.10 % of phages remain un-adsorbed, with cell lysis rate dropped from 0.89 d-1 to 0.26 d-1 compared to the untreated control. The highest phage titer (70 %) was obtained with 1.5 mg/H2O2 pretreated cells. This study emphasizes that low-dose H2O2 eliminates Microcystis but severely affects phage lysis and MCs release depending on H2O2-induced ROS levels. It is a crucial consideration when using phages to control cyanobacterial blooms with H2O2-induced stress.

Assessment of nitrogen interaction with temperature on the growth and toxin production of mat-forming toxin-producing Anagnostidinema carotinosum.

AIMS: Global warming and eutrophication contribute to the severity of cyanobacteria blooms. However, it is unclear how these factors influence the growth and toxin production of Anagnostidinema carotinosum. METHODS AND RESULTS: Based on morphological and molecular analysis, this is the first time A. carotinosum was identified in South Korea. The interactive effect of temperature (25, 30 or 34°C) and nitrogen (2.5, 3.5 or 4.5 mg NO3 -N l-1 ) on A. carotinosum growth and toxin production was studied. Increasing nitrogen limitation reflects reduced growth and chlorophyll-a content at all temperatures. However, the growth was effective under nitrogen limitation when temperatures exceeded 25°C. The maximum growth was found at 30°C, followed by 34°C under higher nitrate levels (3.5 and 4.5 mg l-1 ). In addition, the cell microcystin and anatoxin-a quota increased significantly at 25°C with increasing nitrate limitation, decreasing considerably at 30°C in the same nitrate gradient. CONCLUSION: These results suggested temperatures stimulate A. carotinosum growth at 30 and 34°C and cellular toxin quota at 25 and 34°C with increasing NO3 -N levels. SIGNIFICANCE AND IMPACT OF THE STUDY: These findings imply that limiting nitrogen input alone can effectively reduce biomass; however, controlling A. carotinosum and its toxins at higher temperatures under nitrate limitation is necessary for water quality.

Impact of melatonin on the hydrogen peroxide treatment efficacy in Microcystis aeruginosa: Cell growth, oxidative stress response, and gene transcription.

A study was conducted to determine how melatonin (MLT), a growth regulator, affects Microcystis aeruginosa cell behaviour and how MLT exposed cells respond to hydrogen peroxide (H2O2) treatment. MLT promotes the growth, chl-a content, Fv/Fm values, and microcystins (MCs) production of M. aeruginosa at low concentrations of 1-2.5 µmol/L but suppresses the growth at high concentrations (5-10 µmol/L). The cellular and genetic responses of MLT pre-treated cells to H2O2 treatment were examined further. Further research found that the cells pre-treated with MLT were susceptible to a range of growth-promoting, inhibiting and lethal effects when exposed to higher levels of H2O2. A dose-dependent pattern was observed under conditions of 0.05-0.2 mmol/L H2O2 with 0.5-2.5 µmol/L MLT concentrations to different degrees. High doses of H2O2 (0.2 and 0.3 mmol/L) typically lead to cell lysis and release of MCs in 5.0 and 10 µmol/L MLT pre-treated cells. A decrease in SOD/CAT activities and an increase in MDA levels validated the growth reduction. Furthermore, higher cell lysis and release of intracellular MCs were observed when H2O2 was increased for 5-10 µmol/L MLT pre-treated cells. This led to a higher accumulation of extracellular MCs. The results provide insight into how MLT influences H2O2 damage and assist in identifying situations where H2O2 treatment of cyanobacterial blooms is most appropriate.

Comparative growth characteristics and interspecific competitive interaction of two cyanobacteria, Phormidium autumnale and Nostoc sp. .

This study examined the growth characteristics and competitive interaction of two cyanobacteria, Phormidium autumnale GJ_2B_I1 and Nostoc sp. DS_2B_I1, which were newly isolated from a southeast river (Nakdong) during the cyanobacterial harmful algal bloom (CyanoHAB) season in Korea. As major environmental parameters, water temperature (25 and 30 °C) and alkalinity (19-78 mg CaCO3 L-1 ) and nitrate concentration (1.5-3.5 mg NO3 -N L-1 ) were selected based on the water environmental monitoring data during the CyanoHAB season. Unlike P. autumnale, Nostoc sp. has a relatively high growth rate under both monoculture and co-culture and prefers the maximum environmental conditions (30 °C and 78 mg CaCO3 L-1 ; pH 9) during the CyanoHAB season. In addition, the growth of P. autumnale is relatively unaffected by alkalinity. Nitrogen (N) stress also has a limiting effect in the interspecific interactions of both cyanobacterial strains. All other cases except for Nostoc sp. in a co-culture showed a considerable increase in growth rate with increasing N content (1.5-3.5 mg NO3 -N L-1 ), showing 20-64% under the minimum field conditions (25 °C and 19 mg CaCO3 L-1 ; pH 7) and 18-140% under the maximum field conditions. The results show that the growth of P. autumnale can be stimulated by enhanced N stress. On the other hand, Nostoc sp. is less affected by N stress compared with P. autumnale. Therefore, it has excellent potential to be a major group of CyanoHABs because of their relatively high growth rate, particularly in the range of N tested.

Hormones induce the metabolic growth and cytotoxin production of Microcystis aeruginosa under terpinolene stress.

Several organic compounds released into the aquatic environment have a detrimental impact on humans and other organisms. There is a lack of knowledge about natural hormones and herbicides on non-target organisms, including cyanobacteria. In this study, the response of Microcystis aeruginosa to four phytohormones, indole-3-acetic acid (IAA; 10-5), zeatin (ZT; 10-5), abscisic acid (ABA; 10-7), and brassinolide (BRL; 10-9 mol/L), exposed to terpinolene (TPN; (0.44, 0.88, 1.17, or 1.62 mmol/L) at the cellular and genetic levels were investigated. The results showed that TPN could inhibit the growth and photosynthetic activities and stimulate microcystins (MCs) of M. aeruginosa at various levels through the co-occurrence of oxidative stress, antioxidant defense activities, and an imbalance of the antioxidative system. Hormones played critical roles in the growth promotion and photosynthetic activity by enhancing the antioxidant defense mechanisms and MCs production of M. aeruginosa under TPN stress in both hormone and TPN dose-dependent manner. The growth performance and photosynthetic activities of M. aeruginosa were significant with IAA (p < 0.01) and BSL (p < 0.05) compared to ZT and ABA, as TPN concentrations increased. Hormones stimulated the MCs production significantly BSL (p < 0.05) at various levels and protected the cells against TPN-induced oxidative stress and expression of mcyB and mcyD genes involve in MCs synthesis. Our results indicated that hormone contamination in eutrophic lakes might increase the risk of Microcystis aeruginosa bloom and microcystin production with the TPN association.

Influence of Abiotic Factors on the Growth of Cyanobacteria Isolated from Nakdong River, South Korea1.

Changes in physico-chemical factors due to natural climate variability and eutrophication could affect the cyanobacterial growth patterns in aquatic systems that may cause environmental health problems. Based on morphological and 16S rRNA gene analysis, three cyanobacterial species isolated for the first time from the Nakdong River water sample in South Korea were identified as Amazoninema brasiliense, Microcystis elabens, and Nododsilinea nodulosa. The variations in temperature, pH, nitrogen, or phosphorus levels significantly impacted the cyanobacterial growth patterns. The optimal temperature range for the growth of isolates was from 25-30°C. A neutral or weak alkaline environment favored growth; however, A. brasiliense resulted in 44.2-87.5% higher biomass (0.75 g · L-1 as dry solids, DS) and growth rate (0.24 · d-1 ) at pH 7 than the other isolates (0.4-0.52 g DS · L-1 , 0.16-0.19 · d-1 ). The increased nitrate-nitrogen (NO3 -N) concentrations significantly (P < 0.05) favored biomass production and growth rate for A. brasiliense and M. elabens, respectively, and the maximum growth rate was observed for A. brasiliense at 3.5 mg NO3 -N · L-1 . The orthophosphate concentration (PO4 -P) from 0.1 to 0.5 mg PO4 -P · L-1 increased the growth of the isolates. These observations suggest that isolate growth rates in water bodies can vary depending on different physico-chemical parameters. This study contributes to the further understanding of the growth of microalgae in natural freshwater bodies under fluctuating environmental conditions and aquatic ecosystem stability.

Characterization of Trichoderma asperellum RM-28 for its sodic/saline-alkali tolerance and plant growth promoting activities to alleviate toxicity of red mud.

Red mud (RM) is a highly alkaline, saline and sodic solid by-product released by alumina industries, which pose an economical and environmental problem and establishment of vegetation on these sites is a big challenge. In the present study, a fungus RM-28 exhibiting high tolerance to alkaline (pH 12), saline/sodic (NaCl 4%) was isolated from RM flooded rhizosphere soil of bermudagrass and tested its ability to reduce RM toxicity and promote the growth of sorghum-sudangrass seedlings. This fungus also exhibited high tolerance to heavy metal(loid)s (HMs) and desirable plant growth-promoting traits. This fungus was identified as Trichoderma asperellum based on its internal transcribed spacer (ITS) of rDNA and translation elongation factor-1α (TEF 1α) gene analysis. This fungus was effective in reducing the pH and solubilizing tricalcium phosphate under high alkaline and saline conditions in vitro. Further, RM-28 inoculation significantly lowered the pH and EC of the red mud from 11.8 to 8.2 and 2.23 to 1.42, respectively. Inoculation of RM-28 significantly improved the growth, chlorophyll content and reduced the oxidative stress of sorghum-sudangrass seedlings grown in red mud leachate. These observations suggest that T. asperellum RM-28 serves as potential source for the establishment of vegetation on red mud/red mud contaminated soils.

Penicillium menonorum: A Novel Fungus to Promote Growth and Nutrient Management in Cucumber Plants

The present study is the first report on the isolation of Penicillium menonorum from rhizosphere soil in Korea and its identification based on morphological characteristics and internal transcribed spacer gene sequence. The fungal isolate was named KNU-3 and was found to exhibit plant growth-promoting (PGP) activity through indole acetic acid (IAA) and siderophore production, as well as P solubilization. KNU-3 produced 9.7 mg/L IAA and solubilized 408 mg of Ca3PO4/L, and inoculation with the isolate significantly (p < 0.05) increased the dry biomass of cucumber roots (57%) and shoots (52%). Chlorophyll, starch, protein, and P contents were increased by 16%, 45%, 22%, and 14%, respectively, compared to plants grown in uninoculated soil. The fungus also increased soil dehydrogenase (30%) and acid phosphatase (19%) activities. These results demonstrate that the isolate KNU-3 has potential PGP attributes, and therefore it can be considered as a new fungus to enhance soil fertility and promote plant growth. Moreover, the discovery of PGP ability and traits of this fungus will open new aspects of research and investigations. In this study, plant growth promotion by P. menonorum KNU-3 is reported for the first time in Korea after its original description.

A New Record of Volutella ciliata Isolated from Crop Field Soil in Korea

During a survey of fungal species in South Korea, a species of Volutella ciliata was isolated and described based on the analysis of the internal transcribed spacer region of its rDNA and its morphological characteristics. This is the first record of Volutella ciliata isolated from crop field soil in Korea.

First Report of Mortierella alpina (Mortierellaceae, Zygomycota) Isolated from Crop Field Soil in Korea

A new recorded species of Mortierella was recovered during the investigation of fungal communities in soil samples collected from different locations of Gangwon-do, Korea. The species was identified and described as Mortierella alpina on the basis of phylogenetic analysis of internal transcribed spacer sequences and morphological characteristics. This species has not been officially reported from Korea thus far.